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c myc tag ouse monoclonal antibody  (Proteintech)


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    Structured Review

    Proteintech c myc tag ouse monoclonal antibody
    C Myc Tag Ouse Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 982 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/myc+tag+mab/MYC+tag+Antibody/pm42816460-143-28-34
    Average 96 stars, based on 982 article reviews
    c myc tag ouse monoclonal antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Binding Assay:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    SDS Page:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Recombinant:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Far Western Blot:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Membrane:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Incubation:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Control:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Immunoprecipitation:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Infection:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Stable Transfection:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Co-Immunoprecipitation Assay:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Transfection:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Cell Culture:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Comparison:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Plasmid Preparation:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Western Blot:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Expressing:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Biomarker Discovery:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Knockdown:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Derivative Assay:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    RNA Extraction:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Virus:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Titration:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Sandwich ELISA:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Over Expression:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Inhibition:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Activity Assay:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Mutagenesis:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Staining:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Fluorescence:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Microscopy:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Ubiquitin Proteomics:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Solvent:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Sequencing:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Bioprocessing:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Residue:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Lysis:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Translocation Assay:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Confocal Microscopy:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Software:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Fractionation:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Extraction:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Marker:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Isolation:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Blocking Assay:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    High Molecular Weight:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Luciferase:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Reporter Assay:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).

    Activation Assay:

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation
    Article Snippet: The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239-specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclonal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technology, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described ( ).

    Article Title: GRP75 blocks hepatitis E virus infection by targeting HEV-ORF2 for degradation through chaperone-mediated autophagy and promoting IRF3 activation.
    Article Snippet: The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.The immunization of rabbits and affinity purification of p239specific polyclonal antibody from immunized rabbits were conducted by Genscript Co. Ltd.. The MYC tag Mab (ProteinTech, Wuhan, Hubei, China), anti-GRP75 Mab (Santa Cruz Biotechnology, Santa Cruz, CA, USA), the anti-VDAC1 Mab (ProteinTech), anti-COX4 Mab (ProteinTech), anti-HSC70 Mab (Santa Cruz Biotechnology), anti-LAMP2A polyclo nal antibodies (pAb, Thermo Fisher Scientific, 51-2200) and anti-TBK1 Mab (Santa Cruz Biotechnology), anti-MAVS rabbit polyclonal antibodies pAb, (Cell Signaling Technol ogy, Danvers, MA, USA), anti-GRP75 rabbit pAb (ProteinTech), and anti-Phos-IRF3-S396 rabbit Mab (Cell Signaling Technology) were obtained from commercial suppliers.. The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).The anti-HEV-ORF1 monoclonal antibody (X domain of Sar55 strain) was previously described (65).



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